Antibiotic-resistant bacteria and novel therapeutic options were discussed at the recent international online conference on Infectious Diseases in a One Health context. Organized by the One Health Institute of the Faculty of Health Sciences, the Faculty of Agricultural and Food Sciences and Environmental Management, the Institute of Metagenomics of the University of Debrecen, the Veterinary Medical Research Institute and the Centre for Ecological Research of the Hungarian Research Network, the objective of the event was to promote discussion and knowledge exchange.